《北方園藝》[ISSN:1001-0009/CN:23-1247/S] 卷: 期數(shù): 2024年2 頁碼: 118-124 欄目: 出版日期: 2024-01-31

Title:Analysis of Genetic Diversity and Germination Promoting Effect of Gastrodia elata Germinating Fungi in Hanzhong

文章編號:1001-0009(2024)02-0118-07

作者:彭浩1; 曹丹1; 司豆豆1; 張升明2; 3; 張懷榮4; 李娜1（1.陜西理工大學(xué) 生物科學(xué)與工程學(xué)院，陜西 漢中 723000；2.陜西春森菌業(yè)有限公司，陜西 漢中 723500；3.陜西省西鄉(xiāng)縣食用菌研究所，陜西 漢中 723500；4.寧強(qiáng)縣真菌研究所，陜西 漢中 724400）

Author(s):PENG Hao1; CAO Dan1; SI Doudou1; ZHANG Shengming2; 3; ZHANG Huairong4; LI Na1(1.School of Bioscience and Engineering，Shaanxi University of Technology，Hanzhong，Shaanxi 723000；2.Shaanxi Chunsen Mushroom Industry Co.Ltd.，Hanzhong,Shaanxi 723500；3.Xixiang County Edible Fungi Research Institute，Hanzhong,Shaanxi 723500;4.Ningqiang County Institute of Fungi，Hanzhong,Shaanxi 724400)

關(guān)鍵詞:天麻; 萌發(fā)菌; 拮抗; ITS; 酯酶同工酶

Keywords:Gastrodia elata; germination fungi; antagonism; ITS; esterase isozyme

分類號:Q 939

DOI:10.11937/bfyy.20232599

文獻(xiàn)標(biāo)志碼:A

摘要:以漢中地區(qū)9株天麻萌發(fā)菌及天麻花粉種子為試材，采用拮抗試驗(yàn)、ITS序列測定、酯酶同工酶技術(shù)和種子拌播等方法，研究了9株天麻萌發(fā)菌的遺傳多樣性、親緣關(guān)系和促萌效果，以期為天麻萌發(fā)菌的種質(zhì)資源管理與評價等提供參考依據(jù)。結(jié)果表明：9株萌發(fā)菌根據(jù)兩兩拮抗試驗(yàn)的程度不同分為不顯著、顯著和極顯著3類；根據(jù)ITS序列的差異分為Mycena sp.和Heydenia sp. 2類；根據(jù)酯酶同工酶酶譜條帶在80% 的相似水平上可分為5類；9株萌發(fā)菌與天麻種子共生萌發(fā)，其中C3菌株菌絲生長速度最高為（0.30±0.01）cm·d-1，C2菌株的萌發(fā)率最高為64.66%±2.89%，C2和C3菌株的綜合表現(xiàn)最佳。

Abstract:The genetic diversity，genetic relationship and germination promoting effect of 9 Gastrodia elata germinating fungi and their pollen seeds in Hanzhong area were studied by means of antagonistic experiment，ITS sequence determination，esterase isozyme technology and seed mixing，in roder to provide reference for the management and evaluation of germplasm resources of Gastrodia elata germinating fungi.The results showed that 9 germination fungi were classified into 3 types，insignificant，significant and extremely significant according to the degree of antagonism of each pair；according to the difference of ITS sequence，they were classified into Mycena sp.and Heydenia sp.2；according to esterase isozyme.The zymogram bands could be divided into 5 categories at a similar level of 80%；9 germination strains co-germinate with Gastrodia elata seeds，of which the highest growth rate of C3 strain was (0.30±0.01)cm·d-1，and the highest germination rate of C2 strain was 64.66%±2.89%，the comprehensive performance of C2 and C3 strains were the best.